There are numerous methods of knowing the vicinity of the infection in the human body and out of them ELISA and Western Blot are exceptionally well known today as HIV test. The name of Western blotching, it is additionally called Western smear. ELISA is the shortened form of chemical connected immuno sorbent examine. Western smudging and catalyst connected immuno sorbent test (ELISA) are broadly utilized as a part of the protein identification, and they are particularly utilized as a part of protein recognition. Western smearing and ELISA have application in experimental examines, …